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imagej analysis  (Bio-Rad)


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    Structured Review

    Bio-Rad imagej analysis
    Imagej Analysis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 35689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+images/Image+Lab+Software/pm42042916-170-7-16
    Average 99 stars, based on 35689 article reviews
    imagej analysis - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Software:

    Article Title: 4′-Demethylpodophyllotoxin functions as a mechanism-driven therapy by targeting the PI3K-AKT pathway in Colorectal cancer
    Article Snippet: .. The resulting images were subsequently scanned and partially quantified using ImageJ software (Bio-Rad, Hercules, USA). ..

    Article Title: 4'-Demethylpodophyllotoxin functions as a mechanism-driven therapy by targeting the PI3K-AKT pathway in Colorectal cancer.
    Article Snippet: The treatment of colorectal cancer (CRC) poses significant challenges in terms of drug resistance and poor prognosis, necessitating the exploration of effective therapeutic strategies.. In this study, high-throughput drug screening was utilized to identify Chinese herbal medicines with notable therapeutic effects on CRC.. Among the compounds identified, 4′-demethylpodophyllotoxin (DOP), a derivative of podophyllotoxin, emerged as a potent anti-cancer compound.

    Article Title: IL-33/ST2 axis mediates diesel exhaust particles-induced mast cell activation
    Article Snippet: Images were acquired with an optical microscope (Olympus, Tokyo, Japan) or confocal microscope (Leica, IL, USA). .. The images were analyzed by ImageJ software (Bio-Rad Laboratories, Hercules, CA, USA). ..

    Article Title: IL-33/ST2 axis mediates diesel exhaust particles-induced mast cell activation.
    Article Snippet: Images were acquired with an optical microscope (Olympus, Tokyo, Japan) or confocal microscope (Leica, IL, USA). .. The images were analyzed by ImageJ software (Bio-Rad Laboratories, Hercules, CA, USA). ..

    other:

    Article Title: Reduction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant infection by blocking the epidermal growth factor receptor (EGFR) pathway.
    Article Snippet: The proteins were transferred from the gel to a PVDF membrane (Merck, Millipore, Germany) for 1 h and 20 min, and the membrane was blocked with 5% skim milk in Trisbuffered saline with 2% Tween 20 for 1 h. Primary antibodies diluted in 3% bovine serum albumin (BSA; Thermo Fisher Scientific) and secondary antibodies, such as anti-rabbit and anti-mouse antibodies (GeneTex, Invitrogen, California, USA), diluted in 5% skim milk were used to measure protein expression.

    Article Title: Tim-3 Deficiency Ameliorates Motor Deficits and Neuroinflammation in MPP+/MPTP-Induced Parkinson's Disease Models via the NF-κB/NLRP3 Pathway.
    Article Snippet: Parkinson’s disease (PD) is a common neurodegenerative disorder, and neuroinflammation plays a pivotal role in its pathogenesis.. T-cell immunoglobulin and mucin-domain-containing molecule 3 (Tim-3) is a crucial immunoregulatory mediator in various diseases; however, its roles and underlying molecular mechanisms in PD remain unclear.. We established in vitro and in vivo 1-methyl-4-phenylpyridinium (MPP+)/1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD models in Tim-3–knockout BV2 cells and mice, respectively.

    Article Title: Reduction of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variant infection by blocking the epidermal growth factor receptor (EGFR) pathway
    Article Snippet: The resulting images were analyzed using ImageJ software (Bio-Rad, Hercules, CA, USA) for band quantification.

    Imaging:

    Article Title: DNA:RNA hybrid mutational landscapes reveal a common route to genetic instability in evolving genomes
    Article Snippet: Membranes were incubated with anti DNA:RNA hybrids (S9.6, Kerafast; 0.3 μg/mL in TBS, 0.5% Tween-20, 5% skimmed milk) or anti double-stranded DNA (HYB331-01, Santa Cruz Biotechnology; 1:5,000 in TBS, 0.5% Tween-20, 5% BSA) and revealed using chemiluminescent reagents (Supersignal, Thermo Fisher Scientific) following incubation with anti-mouse peroxidase-conjugated antibodies (Jackson ImmunoResearch). .. Relative DNA:RNA hybrid and total DNA amounts were quantified with ImageJ using images acquired through a ChemiDoc MP Imaging System (Bio-Rad), with serial dilutions of a reference sample as a standard. ..



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    Oxford Instruments imagej images
    a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). <t>ImageJ</t> images processed <t>with</t> <t>Imaris</t> (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.
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    Image Search Results


    a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). ImageJ images processed with Imaris (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.

    Journal: Nature

    Article Title: Fibroblastic reticular cells direct the initiation of T cell responses via CD44

    doi: 10.1038/s41586-025-09988-8

    Figure Lengend Snippet: a – d , Numbers of CD8 T cells ( a ), virus-specific IE1-tetramer + CD8 T cells ( b ) and percentage ( c ) and number ( d ) of MPEC (CD127 hi ) and SLEC (CD127 low ) IE1 + virus-specific CD8 T cells in spleens of uninfected mice or mice infected with wild-type or ∆vCD44BP MCMV (7 dpi). Data in a – d are pooled from three independent experiments (naive, n = 6; MCMV and ∆vCD44BP, n = 12). e , Numbers of cDC1s and cDC2s in spleens of uninfected mice or mice infected with MCMV or ∆vCD44BP. Data are pooled from two independent experiments ( n = 8 per group). f , Frequency of IV − dendritic cells in spleens of uninfected mice or mice at 36 h after infection with MCMV or ∆vCD44BP. Data are pooled from two independent experiments (naive, n = 5; MCMV and ∆vCD44BP, n = 8). g , Spleen sections from uninfected mice and mice infected with MCMV or ∆vCD44BP (2 dpi). ImageJ images processed with Imaris (for display purposes only) are shown. Scale bars, 250 μm. WP, white pulp; MZ, marginal zone; MZBC, marginal zone bridging channel. h , Mean CD11c staining intensities in T cell zones at 2 dpi. i , Representative confocal image showing CD8, IE1 and CD11c in the splenic white pulp of MCMV-infected mice (2 dpi). The white circle highlights an area in which MCMV-infected IE1 + cells are in close contact with CD11c + dendritic cells and CD8 + T cells. The image is representative of data from three independent experiments. Scale bar, 20 μm. All mice were infected with 5 × 10 3 PFU. Graphs show mean ± s.e.m.; violin plots show median and quartiles; significance tested by two-sided Mann–Whitney test.

    Article Snippet: ImageJ images processed with Imaris (for display purposes only) are shown.

    Techniques: Virus, Infection, Staining, MANN-WHITNEY